Antibody and complement-dependent viral neutralization

Vaccinia virus complement-control protein prevents antibody-dependent complement-enhanced neutralization of infectivity and contributes to virulence.

The role of a viral gene product in evasion of the host immune response was investigated. The antibody-dependent complement-enhanced neutralization of vaccinia virus infectivity was prevented by the culture medium from vaccinia virus-infected cells. The vaccinia virus complement-control protein (VCP) was identified as the secreted product of vaccinia virus gene C21L and has homology to a group ...

Complement Neutralization of Herpes Novel Mechanism of Antibody-Independent

Significance of Bivalence of Antibody in Viral Neutralization

The role of bivalence of antibody in its capacity to neutralize virus was studied with rabbit antibodies to the bacteriophage, phiX174. Univalent Fab or Fab' fragments of IgG isolated from antiviral antisera obtained early in the immunization schedule had virtually no activity compared to that of the intact IgG. When the antibodies were isolated from antisera of the same rabbits several months ...

Autophagy-associated dengue vesicles promote viral transmission avoiding antibody neutralization

One of the major defense mechanisms against virus spread in vivo is the blocking of viral infectibility by neutralizing antibodies. We describe here the identification of infectious autophagy-associated dengue vesicles released from infected cells. These vesicles contain viral proteins E, NS1, prM/M, and viral RNA, as well as host lipid droplets and LC3-II, an autophagy marker. The viral RNA ca...

Technique for viral neutralization antibody surveys in primary microcultures.

A simplified, microplate tissue culture procedure is described which facilitates neutralization antibody surveys on large numbers of sera. A comparison of the micromethod with that employing standard tube cultures indicates it to be as sensitive and far more economical.